The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.

Product Description: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail...read more

Product Description: Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques...read more

Product Description: Molecular motor proteins produce force for movement in an incredibly wide variety of cellular processes. This volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family...read more

Product Description: Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study...read more

Product Description: It is now understood that the response of mammalian cells to a wide variety of potentially toxic agents may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer. In Stress Response: Methods and Protocols, Stephen Keyse has assembled a diverse collection of readily reproducible methods devoted to the study of these varied and powerful responses...read more

Offers an introduction to molecular biology techniques in human genome analysis, providing step-by-step instructions for eight experiments utilizing the latest laboratory techniques. (view table of contents)

Product Description: As molecular and cellular biologists move toward nano techniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed...read more

Product Description: As molecular and cellular biologists move toward nano techniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed...read more

Product Description: Practical Skills in Biomolecular Science, is an indispensable book for undergraduate students in the life sciences. The book provides useful support at all stages of a degree course and underpins any practical course in biochemistry, biomedical science, genetics, immunology and microbiology...read more

Product Description: Practical Skills in Biomolecular Science, is an indispensable book for undergraduate students in the life sciences. The book provides useful support at all stages of a degree course and underpins any practical course in biochemistry, biomedical science, genetics, immunology and microbiology...read more

Product Description: Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study...read more

Product Description: This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins...read more

Product Description: A collection of cutting-edge techniques for detecting and quantifying apoptosis, understanding its biochemistry, and for identifying the genes and proteins that regulate and carry it out. Described in step-by-step detail, these readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression...read more

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems.Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:Have you selected the most appropriate research strategy?Have you identified the issues critical to your successful application of a technique?Are you familiar with the limitations of a given technique?When should common procedural rules of thumb not be applied?What strategies could you apply to resolve a problem?A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:Working safely with biological samples and radioactive materialsDNA and RNA purificationPCRProtein and nucleid acid hybridizationProkaryotic and eukaryotic expression systemsProperly using and maintaining laboratory equipment

This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Key Features* Includes a glossary of over 200 terms important for understanding molecular biology* Uses an inexpensive source of eukaryotic cells - great for schools on a budget* Includes Methods Locator that provides instant access to the latest methods* Contain clearly written, easy-to-follow, student-tested instructions:* Sterile techniques* Phage titration* Gel electrophoresis of DNA* Restriction enzyme digestion* Plasmid isolation* Transformation of E. Coli* Recombinant DNA cloning* Nick translation labeling * Nonradioactive primer labelling* Nonradioactive DNA detection* Southern blotting* Colony hybridization* Purification of plant DNA* RNA purification* Northern blotting* Purification of poly A+ RNA* Polymerase chain reaction (PCR)

Product Description: Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed...read more

Product Description: This is the second edition of a highly successful textbook (over 50,000 copies sold) in which a highly illustrated, narrative text is combined with easy–to–use thoroughly reliable laboratory protocols. It contains a fully up to date collection of 12 rigorously tested and reliable lab experiments in molecular biology, developed at the internationally renowned Dolan DNA Learning Center of Cold Spring Harbor Laboratory, which culminate in the construction and cloning of a recombinant DNA molecule...read more (view table of contents, read Amazon.com's description)

Product Description: Expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations...read more (view table of contents, read Amazon.com's description)

Product Description: This revised workbook/lab text consists of 21 projects that can be executed with readily available materials, a minimum of elaborate equipment and a reasonable amount of preparation time. Early projects deal with biochemistry and cytochemistry; the middle ones focus on organelles and their physiology; and later activities explore more advanced molecular topics such as restriction mapping strategies...read more (view table of contents, read Amazon.com's description)

Product Description: This one-semester, project-based laboratory manual gives junior/senior level students the opportunity to characterize the enzyme alpha-amylase. As students proceed through the sequenced experiments, they will learn the principles of DNA, RNA, and protein structure by using modern-day laboratory techniques...read more (view table of contents, read Amazon.com's description)

Product Description: Drawing on state-of-the-art cellular and molecular techniques as well as new and sophisticated imaging and information technologies, this comprehensive, three-volume collection of cutting-edge protocols provides readily reproducible methods for studying and analyzing the events of embryonic development...read more (view table of contents, read Amazon.com's description)

Product Description: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step...read more (view table of contents, read Amazon.com's description)

Product Description: For the increasing number of professionals needing to learn about and use molecular biology techniques in their research, the daunting challenge is to break through the technical and largely inaccessible jargon found in most texts...read more (view table of contents, read Amazon.com's description)